p38α Kinase Enzyme System

产品编号：V2701 品牌：promega 原厂货号：V2701
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
应用分类：

包装：

10μg

运保温度:

-70℃

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描述：

说明		在 Sf9 昆虫细胞中，我们通过杆状病毒表达 N 端带 GST 标签的重组全长人源 p38α 。 p38α （SAPK2A ）是 p38 MAPK 家族的成员，可被多种环境胁迫和前炎性细胞因子激活。 p38 的激活需要通过 MKK （MAP 激酶激酶）将其磷酸化或由 MAP3K7IP1/TAB1 与 p38 的相互作用使其自磷酸化。 p38 的底物包括转录调控因子 ATF2 、 MEF2C 、 MAX 、细胞周期调控因子 CDC25B 以及肿瘤抑制因子 p53 ，体现了该激酶在胁迫相关的转录、细胞周期调控、以及基因毒性胁迫应答方面的作用。 ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： p38α ， 10μg ( 人源，重组全长蛋白 ) ； MW : ~ 67 kDa 。 • 底物： p38 peptide 。 • 其它组分：反应缓冲液， DTT 。 p38α NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 • 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂ 。 • 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		p38 alpha Kinase Enzyme System Product Information
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于 –70°C ；其他组分储存于 –20°C 。
专利/免责声明		For Cat.# V9591: Patents Pending. U.S. Pat. No. 7,700,310 and other patents and patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584 and other patents and patents pending. U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents and patents pending. U.S. Pat. No. 7,741,067 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant full-length human p38α was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38α (SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines. The activation of p38 requires its phosphorylation by MAP kinase kinases (MKKs) or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of p38 include transcription regulators ATF2, MEF2C and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress-related transcription and cell cycle regulation as well as in genotoxic stress response.

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: p38α, 10μg (Human, recombinant full-length). MW: ~67kDa.
Substrate: p38 peptide.
Other: Reaction Buffer, DTT.

p38α NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.