ERK2 Kinase Enzyme System

产品编号：V1962 品牌：promega 原厂货号：V1962
产品分类：蛋白质检测与分析 > 酶检测和活性分析 > 激酶检测
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包装：

1mg

运保温度:

-70℃

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描述：

说明		我们在大肠杆菌（E. coli ）细胞中表达 N 末端带 GST 标签的重组全长人源 ERK2 ，该蛋白在体外可被 MEK1 激活。 ERK2 是一个丝/ 苏氨酸蛋白激酶，属于胞外信号调控激酶（ERKs）的成员，被许多生长因子和细胞因子激活；其激活需要 MEK 介导的酪氨酸和苏氨酸的磷酸化。 ERK2 广泛分布于许多组织中，在心脏、大脑和脊髓中表达量最高。激活的 ERK2 转运到细胞核内，磷酸化多种转录因子（如Elk-1 、 c-Myc 、c-Jun 、c-Fos 以及 C/EBP β）。 ADP-Glo™ Kinase Assay （ADP-Glo™ 激酶检测试剂盒）是一种发光法激酶检测试剂盒，用于检测激酶反应产生的 ADP 含量： ADP 转化为 ATP 后， ATP 即可作为 Ultra-Glo™ 萤光素酶催化反应的底物，产生光信号。发光信号与 ADP 的量和激酶活性正相关。该试剂盒适合检测化合物对纯化的多种激酶活性的影响，使之成为初筛和激酶选择性概况分析的理想工具。 ADP-Glo™ 激酶检测试剂盒可用于检测几乎所有可生成 ADP 的酶类 ( 如激酶或 ATP 酶 ) 的活性， ATP 浓度可高达 1 mM 。激酶酶系统的组成： • 激酶： ERK2 ， 10μg ( 人源，重组全长蛋白 ) ； MW : ~ 68 kDa 。 • 底物：天然的猪髓磷脂碱性蛋白（MBP ）。 • 其它组分：反应缓冲液， DTT 。 ERK2 NCBI 数据库入口。人类激酶酶系统及其应用。
特点		• 在实验室内即可筛选更多的化合物： ADP-Glo™ 激酶检测试剂盒与激酶酶系统的优化组合，使您可以迅速方便地开始实验。 • 完整的系统：激酶酶系统包含重组激酶、激酶的最适底物、反应缓冲液、以及使用前需加入缓冲液的 DTT 和 MnCl₂ 。 • 可得到可靠结果： ADP-Glo™ 激酶检测试剂盒提供了宽广的动态范围、方便的操作方法、以及更好的灵敏度，产生的数据更可靠。
操作手册		ERK2 Kinase Enzyme System Product Information
储存条件		收到试剂盒后，请将激酶轻微离心后分装成小份，储存于–70°C ；其他组分储存于–20°C 。
参考文献		Boulton, T.G. et al. (1991) Biochemistry 30(1) , 278–86.
专利/免责声明		For Cat.# V9291: Patents Pending. U.S. Pat. No. 7,700,310 and other patents and patents pending. U.S. Pat. Nos. 6,602,677 and 7,241,584 and other patents and patents pending. U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents and patents pending. U.S. Pat. No. 7,741,067 and other patents and patents pending. The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

原厂资料：

Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs), which are activated in response to numerous growth factors and cytokines. Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta).

ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

Kinase Enzyme System contains:

Kinase: ERK2, 10μg (Human, recombinant full-length). MW: ~68kDa.

Substrate: Native Swine Myelin Basic Protein (MBP).

Other: Reaction Buffer, DTT.

ERK2 NCBI Database Entry.

Human Kinase Enzyme Systems and Applications.

Features - Benefits

Profile More Compounds In-House: ADP-Glo™ Kinase Assay + Kinase Enzyme System is optimized so that you are up and running in no time.
Complete Systems: The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer, DTT and supplemental reagents as needed.
Obtain Reliable Results: The broad dynamic range, the ease of use and better sensitivity obtained with ADP-Glo™ Kinase Assay result in less ambiguous data.

Notes

Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.

References

Boulton, T.G. et al. (1991) Biochemistry 30(1), 278–86.