胃蛋白酶( Pepsin )优先在苯丙氨酸、亮氨酸、酪氨酸和色氨酸的 C 端进行水解。该蛋白酶可单独或与其他蛋白酶联合在质谱分析和其他应用中进行蛋白分析。
应用
•多肽图谱。
• 蛋白鉴定。
• 检测翻译后修饰。
胃蛋白酶
原厂资料:
Pepsin preferentially cleaves at the C-terminus of phenylalanine, leucine, tyrosine and tryptophan. This protease can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Pepsin activity is optimal at pH 1.0–3.0.
Applications
Peptide mapping.
Protein identification.
Characterization of post-translational modifications.