The DNA 5´ End-Labeling System is a complete system for phosphorylating both double- and single-stranded DNA and RNA with T4 Polynucleotide Kinase and [γ-32P]ATP. The system includes enzymes, buffers and control DNA standards to measure reaction efficiencies. Calf Intestinal Alkaline Phosphatase is included for removal of the 5´-phosphate prior to labeling with T4 Polynucleotide Kinase.
Features - Benefits
Convenient:Can use to label both single-stranded and double-stranded DNA and RNA.
Complete:System includes enzymes, buffers and control DNA standards for measuring reaction efficiencies (except radionucleotides).
Flexible:Works with [γ-32P]ATP, [γ-33P]ATP or [γ-35S]ATP.
Applications
Preparation of hybridization probes.
Preparation of sequencing primers.
Labeling marker DNAs and RNAs for autoradiography.