The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of β-galactosidase in reporter gene assays.
• The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors. • Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range. • Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays. • Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions. • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes. • Assay can be completed in about one hour, providing fast assay turnaround.
Sensitive This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
Ready-to-Use The Galacto-Light™ Plus™ system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.
Products described herein are guaranteed for 12 months from the date of purchase.
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For Research Use Only. Not for use in diagnostic procedures.
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