DharmaFECT Duo Transfection Reagent

产品编号：T-2010-03 品牌：Dharmacon 原厂货号：T-2010-03
产品分类：细胞,细胞培养与转染试剂 > 转染试剂 > 非脂质体转染试剂
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包装：

1.5 mL

运保温度:

4 C

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描述：

为siNRA和质粒共转染所优化的高效、超低毒性转染试剂DharmaFECT Duo是一种全新的可高效共转染siRNA和质粒的转染试剂。而上述DharmaFECT 1,2,3和4则仅是针对siRNA单独转染的优化产品。虽然共转染siRNA和质粒进行报告基因系统研究越来越普遍，但对应的能够高效共转染siRNA和质粒的转染试剂则很匮乏；大部分的研究者依然运用传统的质粒转染试剂进行siRNA转染或者siRNA和质粒的共同转染，但是这些试剂没有经过转染条件的优化，往往对细胞活力产生很大的毒副作用。基于此，Dharmacon公司推出的具有在保持细胞高活力的情况下高效共转染siRNA和质粒的DharmaFECT Duo转染试剂。

在一对一的共转染比较实验中，唯独DharmaFECT Duo转染试剂比其它任何转染试剂有更高的siRNA和质粒的共转染效率，具体表现为被转染质粒的高效表达和siRNA所诱导基因的高效沉默（见下图）。

Figure. DharmaFECT Duo achieves optimal co-transfection results - effectiv plasmid delivery (black bars) and target mRNA knockdown (blue bars) when compared to other lipid reagents. The psiCHECK 2 Vector (Promega; 100 ng/well) and siRNA targeting renilla luciferase (100 nM) were complexed with a range of transfection reagent volumes (0.05 - 0.6 μL/well) to determine optimal co-transfection conditions (shown). Firefl y Luciferase expression was assessed at 48h using the Dual-Glo™ Luciferase Assay System (Promega). Cell viability was assessed using the alamarBlue® Assay (Biosource International). siRNA-induced Renilla luciferase knockdown (blue bars) was assessed by branched DNA assay (Panomics).

DharmaFECT Duo转染试剂在众多细胞系的实验结果均表现出众，下面列举出几种常见细胞系转染后的细胞活力和基因沉默效果检测。

表. DharmaFECT ® Duo 转染试剂在众多细胞系的卓越性能表现（96孔细胞培养板）

细胞系	细胞活力测定 (%)	靶基因沉默效果 (%)	转染细胞密度 (cells/mL)	转染试剂使用体积(μL/well)
ES-D3	87	97	2.0×10⁵	0.3
HeLa	79	81	2.0×10⁵	0.1
HepG2	72	91	2.0×10⁵	0.05
Jurkat	89	96	6.0×10⁵	0.6
MCF7	80	99	1.0×10⁵	0.2
MCF10a	78	98	1.0×10⁵	0.2
NIH/3T3	83	97	1.0×10⁵	0.4

原厂资料：

In side-by-side co-transfection experiments with traditional reagents developed for plasmid delivery, only Thermo Scientific DharmaFECT Duo Transfection Reagent resulted in efficient cell delivery of both siRNA (assayed by target gene knockdown) or microRNA reagents, along with plasmid (assayed by level of reporter expression). DharmaFECT Duo is an excellent choice for experiments requiring co-transfection, such as RNAi rescue or utilization of reporter systems.

Highlights
• Effective delivery of two distinct molecules, DNA plasmid and small RNA, from a single formulation
• Efficient transfection without compromising cell viability
• Potent RNAi results together with plasmid expression

For transfection of plasmid DNA or shRNA, please select Thermo Scientific TurboFect Transfection Reagent. For transfection of siRNA or microRNA only, please select Thermo Scientific DharmaFECT 1 Transfection Reagent.