The Complete Kit for Human Whole Blood CD34+ Cells 是一个从全血或者血沉棕黄层中分离CD34+细胞的简单的两步法。首先,用造血祖细胞分离的CD34+细胞可直接用于下游的应用。
原厂资料:
Description
The Complete Kit for Human Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat.
First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail (15186C) with antibodies recognizing CD2, CD3, CD14, CD16, CD19, CD24, CD56, CD66b and CD61 surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18066C), which contains an antibody recognizing CD34. RosetteSep™ binds unwanted cells to red blood cells (RBCs), forming immunorosettes, which sediment during density gradient centrifugation. The pre-enriched fraction containing CD34+ cells is harvested from the interface between the plasma and density gradient medium. The pre-enriched CD34+ cells are then labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated CD34+ cells are immediately available for downstream applications.
Advantages:
• Fast and easy-to-use
• Up to 98% purity
• No columns required
• Can be combined with SepMate™ for consistent, high-throughput sample processing
Product Type:
Cell isolation products
Recommended For:
Positive selection of CD34+ cells from fresh whole blood or freshly prepared buffy coat that contains RBCs at a ratio of at least 30 - 50, and preferably 100, RBCs per nucleated cell.
Components:
• RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 3 x 2.0 mL
• EasySep™ Human CD34 Positive Selection Cocktail, 0.4 mL
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
注意事项:
Hazards Identification
Emergency Overview: This product is a potential irritant to eyes, respiratory system, and skin. This product may also be harmful if ingested. Complete toxicological properties have yet to be determined.
Routes of Exposure: Absorbed through skin, eye contact, inhalation, and ingestion.
Potential Health Effects:
Eye: May cause eye irritation.
Skin: May cause skin irritation.
First Aid Measures
Eyes: In case of contact with eyes, flush thoroughly with water. Call a physician.
Skin: In case of contact with skin, wash the affected area with soap and copious amounts of water. Should irritation occur, contact a physician.
Ingestion: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
Inhalation: If inhaled, remove person to fresh air. If breathing becomes difficult, call a physician.
Puncture Wounds: Wash thoroughly with soap and water. Allow to bleed freely. Call a physician.
Note to Physician: Not Available
3.3.3 Inhalation: May be harmful if inhaled. Material may be irritating to mucous
membranes and upper respiratory tract.
3.3.4 Ingestion: May be harmful if swallowed.
3.4 Chronic Effects/Carcinogenicity: Not Available