The RosetteSep™ HLA B Cell Enrichment Cocktail试剂盒是通过阴选法从全血中分离B细胞。全血中多余的非目标细胞是通过其四聚物的抗体复合物识别C D2、CD3、CD4、CD16、 CD36、CD56、CD66b、CD71及血型糖蛋白A等去除的。纯化的B细胞主要分布在血浆与浮力密度介质之间。得到的目的细胞可以被用来进行血清学研究或流式细胞仪试验分析。
原厂资料：
Description
The RosetteSep™ HLA B Cell Enrichment Cocktail is designed to isolate B cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD16, CD36, CD56, CD66b, CD71 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified B cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The desired cells are immediately ready for serology or flow cytometry crossmatch assays.
This product has been CE Marked in the European Union according to the 98/79/EC In Vitro Diagnostic Device Directive and complies with its requirements.
Application:
In vitro diagnostic
Product Type:
Cell isolation products
Recommended For:
The isolation of human B cells from whole blood by negative selection for HLA analysis
Components:
RosetteSep™ HLA B Cell Enrichment Cocktail
Accessory Products:
• Lymphoprep™ (Catalog #07801), or
• RosetteSep™ DM-L (Catalog #15705)
Area of Interest:
HLA
Cell Isolation Product Type:
Reagents
Cell Source:
Buffy coat, Whole blood
Cell Type:
B cells
Popular Product Line:
RosetteSep™
Selection Method:
Negative
Species:
Human
Intended Use Statement:
FOR PROFESSIONAL IN VITRO DIAGNOSTIC USE ONLY.
注意事项：
Hazards Identification
Emergency Overview: This product is a potential irritant to eyes, respiratory system, and skin. This product may also be harmful if ingested. Complete toxicological properties have yet to be determined.
Routes of Exposure: Absorbed through skin, eye contact, inhalation, and ingestion.
Potential Health Effects:
Eye: May cause eye irritation.
Skin: May cause skin irritation.
First Aid Measures
Eyes: In case of contact with eyes, flush thoroughly with water. Call a physician.
Skin: In case of contact with skin, wash the affected area with soap and copious amounts of water. Should irritation occur, contact a physician.
Ingestion: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
Inhalation: If inhaled, remove person to fresh air. If breathing becomes difficult, call a physician.
Puncture Wounds: Wash thoroughly with soap and water. Allow to bleed freely. Call a physician
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
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