The RosetteSep™ Human B Cell Enrichment Cocktail通过应选的方法从从全血中分离B细胞。全血中多余的非目标细胞是通过其四聚物的抗体复合物识别CD2、CD3、 CD19、CD16、 CD36、CD56、CD66b及血型糖蛋白A等去除的。纯化的B阳性细胞主要分布在血浆与浮力密度介质之间。
原厂资料：
Description
The RosetteSep™ Human B Cell Enrichment Cocktail is designed to isolate B cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD16, CD36, CD56, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified B cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium.
Product Type:
Cell isolation products
Recommended For:
The isolation of human B cells from whole blood by negative selection
Components:
RosetteSep™ Human B Cell Enrichment Cocktail
Accessory Products:
• Lymphoprep™ (Catalog #07801), or
• RosetteSep™ DM-L (Catalog #15705)
Cell Type:
B cells
Popular Product Line:
RosetteSep™
Area of Interest:
Immunology
Species:
Human
Cell Source:
Buffy coat, Whole blood
Selection Method:
Negative
Cell Isolation Product Type:
Reagents
Legal Statement:
Ficoll-Paque™ PLUS is a trademark of GE Healthcare Ltd.
Intended Use Statement:
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
注意事项：
Hazards Identification
Emergency Overview: This product is a potential irritant to eyes, respiratory system, and skin. This product may also be harmful if ingested. Complete toxicological properties have yet to be determined.
Routes of Exposure: Absorbed through skin, eye contact, inhalation, and ingestion.
Potential Health Effects:
Eye: May cause eye irritation.
Skin: May cause skin irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Ingestion: May be harmful if swallowed.
Chronic Effects/Carcinogenicity: Not Available
OSHA Regulatory Status: Not Available
First Aid Measures
Eyes: In case of contact with eyes, flush thoroughly with water. Call a physician.
Skin: In case of contact with skin, wash the affected area with soap and copious amounts of water. Should irritation occur, contact a physician.
Ingestion: If swallowed, wash out mouth with water provided person is conscious. Call a physician.
Inhalation: If inhaled, remove person to fresh air. If breathing becomes difficult, call a physician.
Puncture Wounds: Wash thoroughly with soap and water. Allow to bleed freely. Call a physician.
Accidental Release Measures
Leak and Spill Procedure:Wear chemical-resistant gloves. Absorb spill and place in closed container for disposal. Ventilate and wash area thoroughly after clean-up is complete.
Handling and Storage
Handling: Should be handled by trained personnel observing good laboratory practices. Avoid
breathing vapor. Avoid contact with eyes, skin and clothing.
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