The StemSep™ Mouse CD4+ T Cell Enrichment Kit is designed to isolate CD4+ T cells from single cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-CD4+ T cells (CD8, CD11b (Mac-1), CD45R/B220, Ly-6G/C (Gr-1), TER119) and Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are then separated and StemSep™ Columns and Magnets.
Product Type:
Cell isolation products
Recommended For:
The isolation of mouse T cells from single cell suspensions of splenocytes by negative selection
Components:
• StemSep™ Mouse CD4+ T Cell Enrichment Cocktail, 700 μL (5 x 700 μL)
• Anti-Biotin TAC, 2.0 mL (5 x 2.0 mL)
• StemSep™ Magnetic Colloid, 1.5 mL (5 x 1.5 mL)
• Rat Serum, 2.0 mL (5 x 2.0 mL)
Equipment Required:
• StemSep™ Magnet (Catalog #11030, 11050, 11060, or 11070) or a magnet with the strength of at least 0.5 Tesla
• StemSep™ Negative Selection Columns
Cell Type:
T cells, CD4+ T cells
Popular Product Line:
StemSep™
Area of Interest:
Immunology
Species:
Mouse
Cell Source:
Spleen, Other
Selection Method:
Negative
Cell Isolation Product Type:
Reagents
Intended Use Statement:
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS
注意事项：
Hazards Identification
Emergency Overview: This product is a potential irritant to eyes, respiratory system, and skin. This product may also be harmful if ingested. Complete toxicological properties have yet to be determined.
Routes of Exposure: Absorbed through skin, eye contact, inhalation, and ingestion.
Potential Health Effects:
Eye: May cause eye irritation.
Skin: May cause skin irritation.
Inhalation: May be harmful if inhaled. Material may be irritating to mucous membranes and upper respiratory tract.
Ingestion: May be harmful if swallowed.
3.4 Chronic Effects/Carcinogenicity: Not Available
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