FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
注意事项：
CLUMPY SAMPLES
Q. ARE THERE ANY SUGGESTIONS FOR CLUMPY SAMPLES?
A. Yes. There are several reasons a sample might clump.
1) If the cells were frozen or poorly stored then DNA from dead cells can form clumps. If this is the case adding DNAse to the cell suspension may help.
2) The presence of Ca++/Mg++ in the medium may cause cells, such as monocytes, to clump. Ca++/Mg++ free PBS should be used. EDTA can also be used to decrease clumping.
3) Cells may clump if they become activated. Keeping the cells at 4°C or on ice can prevent this.
Q. MY CORD BLOOD SAMPLE IS CLUMPING. WHAT IS HAPPENING?
A. Cord blood can frequently be “clumpy”. Often, this is a platelet problem, so the slow spin procedure outlined in the StemSep™ Instruction Manual (pg. 33) should be followed.
Q. WHICH ANTICOAGULANT SHOULD BE USED?
A. ACD is better than heparin as it is less likely to give a clumpy or sticky cell suspension.
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