Thermo Scientific Transcription Promoter Sequencing Primers are single-stranded oligonucleotides with 5'- and 3'-hydroxyl ends. The primers are complementary to the SP6, T3, or T7 RNA polymerase promoter regions respectively. Primers are supplied as 10 µM aqueous solutions.
Applications
Sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in common cloning vectors, such as pTZ19R, pTZ57R, and pBluescript II
Quality Control
Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence.
Storage Condition
-20 C
Hazardous
No
原厂资料：
注意事项：
Primers cannot be used for certain plasmids that contain truncated, but still fully functional promoters. Primers are not phosphorylated.
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