Thermo ScientificphiX174 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme, then purified and dissolved in storage buffer. The DNA fragments contain blunt or sticky ends, depending on the restriction enzyme used for the markers preparation.
Thermo Scientific phiX174 DNA/BsuRI (HaeIII) Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols in Resources
Highlights
Sizing and approximate quantification of small DNA fragments
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)
10mM Tris-HCl (pH7.6) and 1mM EDTA.
原厂资料：
注意事项：
Bands indicated ** form an anomalous pattern on polyacrylamide gels.
The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of the respective DNA sequences.
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