SYTOX™ Orange Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 532 nm with an emission profile similar to R-PE, and has been tested in an assortment of cell types.
You get these advantages with SYTOX™ Orange Dead Cell Stain:
• Bright Signal—Easily distinguishes dead cells from live cells. • Rapid Staining—Single step, No Wash Assay to assess viability of unfixed cells. • Validation—in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood. • Optimization—Dye concentration has been optimized for use in flow cytometry. • Versatility—Tested in a variety of experimental applications.
A bright stain in an easy-to-use, no-wash assay SYTOX™ Orange Dead Cell Stain is a cell-impermeant nucleic acid stain. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ Orange. Because it is non-fluorescent in aqueous media, SYTOX™ Orange Dead Cell Stain can be applied to cells and visualized with minimal background staining and without an additional wash step.
Validated in flow cytometry SYTOX™ Orange Dead Cell Stain is designed for flow cytometry using the 488 nm blue laser or 532 nm green laser with emission in the 575/26 BP filter (similar to PE). The dye concentration has been optimized for samples with concentrations ranging from 1x104to 1x106 cells per ml.
SYTOX™ Orange Dead Cell Stain has been validated in several cell types including: • Jurkat • ReH • CHO • Kg1a • 3T3 • HeLa • HEK (primary cells) • Lysed whole blood
SYTOX™ Orange Dead Cell Stain has been tested in several applications including: • Apoptosis • Cell cycle • Cell proliferation • Immunophenotyping assays
Part of the SYTOX™ family of cell viability stains SYTOX™ Dead Cell Stains come in an assortment of excitation/emission characteristics and have been incorporated into a number of assays for apoptosis, cell viability, and metabolism. • SYTOX™ Blue Dead Cell Stain (S34857): 405 nm violet laser.
• SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10309): 488 nm laser with red emission.
• SYTOX™ Red Dead Cell Stain (S34854): 633/5 nm red laser.
• SYTOX™ Green Dead Cell Stain (S34861): 488 nm laser—green emission.
• SYTOX™ Dead Cell Stain Sampler Kit (S34862)
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For Research Use Only. Not for use in diagnostic procedures.