Alexa Fluor® 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor® dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor® dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.
Important Features of Alexa Fluor® 647 Streptavidin Conjugates: • Alexa Fluor® 647 streptavidin conjugate has Ex/Em maxima of ~ (650/668) • Bright, photostable fluorescence • High solubility in aqueous solutions • Available in multiple colors • Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more
Properties of Alexa Fluor® Dyes Alexa Fluor® dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.
Blocking Endogenous Biotin Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.
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For Research Use Only. Not for use in diagnostic procedures.
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