The R-phycoerythrin (R-PE) conjugate of streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (R-phycoerythrin). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Streptavidin R-PE is very valuable in biotin-streptavidin detection protocols developed for flow cytometry, microplate assays, and microarrays. This R-PE streptavidin conjugate is a high-purity product (purity confirmed by HPLC) and is supplied as 1 mL of a 1 mg/mL solution.
Important Features of Streptavidin RPE: • Ex/Em maxima of ~ (496, 546, 565/578) nm • 5 to 10 times brighter than organic dyes • Very high solubility in aqueous solutions • Free of all unconjugated streptavidin; and mostly free of unconjugated R-PE • Ideal for flow cytometry, microplate assays, and microarrays
Properties of R-phycoerythrin R-PE has a quantum efficiency of 78%, making it an incredibly bright reagent, useful where high sensitivity is needed. Because of poor photostability, however, it is not recommended for fluorescence microscopy.
Find Biotinylated Conjugates We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies. • Use the primary antibody search tool to find biotinylated primary antibodies • Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies
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For Research Use Only. Not for use in diagnostic procedures.
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