The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ~496,565/627 and is widely used to detect biotinylated probes by flow cytometry. Not only are the Alexa Fluor™ 610 conjugates of R-PE are more fluorescent than the commercially available Texas Red™ conjugates of R-PE, the fluorescent emission of Alexa Fluor™ 610-R-PE tandem conjugates is shifted to somewhat longer wavelengths than is the emission of Texas Red™-R-PE conjjgates, resulting in better separation from the emission of R-PE.
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