3′-O-Me-m7G(5)ppp(5)G RNA Cap Structure Analog

• 产品编号：S1411S      品牌：New England Biolabs       原厂货号：S1411S
• 产品分类：PCR/RT-PCR/qRT-PCR > 其它 > 核苷酸 > 帽相似物&变异型（Cap Analog & Variants）
• 应用分类：

描述：

Blocking of the 3´ -hydroxyl of m⁷G with 3´-0-Me assures that the capped transcripts are homogeneous. The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation (1). 

The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (2,3,4). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (5), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (5,6). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (6). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (7).

A method using E. coli RNA Polymerase primed with m⁷G(5´)ppp(5´)G or m⁷G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (8). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA Polymerase primed with m⁷G(5´)ppp(5´)G (7).
 

Sequence

3'-O-Me-7mG(5')ppp(5')G

Supplied As

Lyophilized Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

原厂资料：