HMGS-2 PMA-FREE 5ML PMA FREE 5 ML

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5ml bottle of HMGS-2 contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of medium.

When a 500 ml bottle of medium is supplemented with HMGS-2, the final concentrations of the components in the supplemented medium are:

• Fetal bovine serum (0.5%)
• Basic fibroblast growth factor (3 ng/ml)
• Bovine pituitary extract (0.2%)
• Heparin (3 µg/ml)
• Hydrocortisone (0.18 µg/ml)
• Recombinant human insulin-like growth factor-I (1 µg/ml)
• Transferrin (5 µg/ml)
• Endothelin-1 (10 nM)