Human TIMP-1 ELISpot Development Module, 5 Plate (1 KT)

• 产品编号：RD-SEL970      品牌：R&D       原厂货号：SEL970
• 产品分类：ELISA及其它免疫分析 > ELISpot及FluoroSpot > ELISpot试剂盒
• 应用分类：

描述：

Molecule Information:  TIMP-1
Long Name: Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs: 7076 (Human); 21857 (Mouse); 116510 (Rat)
Background: TIMPs 
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

原厂资料：

Molecule Information:  TIMP-1
Long Name: Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs: 7076 (Human); 21857 (Mouse); 116510 (Rat)
Background: TIMPs 
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

注意事项：

* Sold separately (see Other Reagents Required, below)
** Following an overnight incubation with capture antibody, 2 hour blocking step, and cell stimulation.
*** When using the protocol provided.