Interleukin 2 (IL-2) was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has also been shown to stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. At the amino acid sequence level, there is approximately 60% - 90% similarity between species. Mature human IL-2 shows 65%, 67%, 72%, 78%, and 64% aa identity to mouse, rat, pig, cat, and cow IL-2, respectively.
原厂资料:
Product Summary:
The Quantikine Rat IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural rat IL-2 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-2.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of rat IL-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=14)
96
83-114
Heparin Plasma (n=4)
100
92-116
Cell Culture Supernates (n=8)
110
93-120
EDTA Plasma (n=4)
102
90-117
Linearity:
To assess the linearity of the assay, four or more samples containing and/or spiked with various concentrations of rat IL-2 were diluted with Calibrator Diluent and then assayed.