Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper (Th) 2 clones that inhibited cytokine production by Th1 clones . The human ortholog of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% identical at the nucleotide and amino acid level. IL-10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. A number of viruses, including Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), also encode viral members of the IL-10 family. Human IL-10 has cross-species actvity and is active on mouse cells. Mouse IL-10 is species-specific and does not act on human cells.
原厂资料：
Product Summary:
The Quantikine Rat IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-10 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-10 accurately. Results obtained using natural rat IL-10 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-10.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
138
270
660
132
270
682
Standard Deviation
6.3
9
16.1
13.1
19.7
59.8
CV%
4.6
3.3
2.4
9.9
7.3
8.8
Cell Culture Supernates
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
103
218
582
114
236
598
Standard Deviation
5.7
9.5
17.2
10.8
16.4
42.6
CV%
5.5
4.4
3
9.5
6.9
7.1
Recovery:
The recovery of rat IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=6)
94
82-112
Cell Culture Supernates (n=5)
106
96-118
EDTA Plasma (n=6)
100
88-115
Linearity:
To assess the linearity of the assay, five or more samples spiked with various concentrations of rat IL-10 in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
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