IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
原厂资料：
Product Summary:
The QuantiGlo Human IL-1 beta Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-1 beta and antibodies raised against the recombinant protein. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 beta showed linear curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-1 beta. Reports indicate that this and other ELISA kits calibrated using mature IL-1 beta as a standard will detect, but considerably underestimate, the unprocessed IL-1 beta precursor present in samples. In biological samples other than cell lysates, the precursor form of IL-1 beta is usually not the predominant form of IL-1 beta present and, additionally, is not biologically active. Therefore, results obtained using this kit should provide a useful measure of the levels of active IL-1 beta present in biological fluids.
Precision:
Intra-Assay Precision (Precision within an assay)Four samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Four samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of IL-1 beta spiked to three different levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
106
100-115
Serum (n=4)
96
87-102
EDTA Plasma (n=4)
94
88-107
Heparin Plasma (n=4)
93
87-103
Citrate Plasma (n=4)
95
90-101
Linearity:
To assess the linearity of the assay, four samples containing or spiked with high concentrations of IL-1 beta in various matrices were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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