IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins.
原厂资料：
Product Summary:
The Quantikine Porcine IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mature porcine IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant porcine IL-1 beta. Results obtained using natural porcine IL-1 beta show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-1 beta.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
136
348
780
141
338
783
Standard Deviation
9.8
17
40.9
12.2
20.5
31.7
CV%
7.2
4.9
5.2
8.7
6.1
4
Recovery:
The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
106
102-109
Serum (n=4)
111
102-117
Heparin Plasma (n=4)
111
98-117
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
