Long Name: Signal Transducer and Activator of Transcription 1
Aliases: CANDF7; ISGF-3; STAT91
Entrez Gene IDs: 6772 (Human); 20846 (Mouse)
Background: STAT1
The STAT family of transcription factors, first described as key mediators of interferon signal transduction, are now recognized as signaling factors downstream of many cytokines, growth factors, and hormones. At least seven STATs exist: STAT1, 2, 3, 4, 5a, 5b, and 6. They are responsible for an array of cellular activities including regulating growth, survival, differentiation, motility, and the immune response. They are characterized structurally by the presence of the Src homology 2 (SH2) domain, and a C-terminal tyrosine phosphorylation site. Upon ligand binding, they are recruited to the activated receptor, via their SH2 domains, to receptor-associated tyrosine kinases of the Janus kinase family (JAK). Once phosphorylated by JAK, STATS are free to hetero- or homo-dimerize, and translocate to the nucleus where they bind DNA to regulate gene transcription.
STAT1 is associated with type I and II interferon signaling. Phosphorylation of STAT1 at Y701 leads to dimerization and translocation to the nucleus to activate gene transcription. Human STAT1 shows 93% and 94% aa identity with mouse and rat STAT1, respectively, over the region used as an immunogen. This region is identical between isoforms STAT1a (91 kDa) and STAT1b (84 kDa).
原厂资料:
Specificity:Detects human and mouse STAT1 p91 in Western blots. In Western blots, less than 1% cross-reactivity with human or mouse STAT1 p84 is observed. Additionally, no cross-reactivity with the other STATs has been detected by immunoprecipitation or Western blotting.
Applications:
Please note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
3 µg/106 cells
See below
Western Blot
Detection of Human and Mouse STAT1 p91 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for STAT1 p91 at approximately 91 kDa (as indicated). This experiment was conducted under reducing conditions and usingImmunoblot Buffer Group 1.
Immunoprecipitation
Immunoprecipitation of Human STAT1. STAT1 p91 was immunoprecipitated from 200 µg of cell lysate of HeLa human cervical epithelial carcinoma cell line following incubation with 3 µg Goat Anti-Human/Mouse STAT1 p91 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST1) overnight at 4 °C. STAT1 p91-antibody complexes were absorbed using Protein G sepharose (Invitrogen, Catalog # 10-1242). Immunoprecipitated STAT1 p91 was detected by Western blot using 1 µg/mL Human STAT1 Monoclonal Antibody (Catalog # MAB14901). View ourrecommended buffer recipes for immunoprecipitation.