Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
原厂资料：
Product Summary:
The Quantikine Porcine IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure porcine IL-12/IL-23 p40 in cell culture supernates, serum and plasma. It contains CHO cell-expressed recombinant porcine IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural porcine IL-12/IL-23 p40 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring porcine IL-12/IL-23 p40.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
27
27
27
Mean
89.8
362
819
88.8
361
795
Standard Deviation
7.3
17.7
28
9.2
22.9
46.1
CV%
8.1
4.9
3.4
10.4
6.3
5.8
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
25
25
25
Mean
115
447
991
110
454
995
Standard Deviation
7.4
22.7
33
10.1
33.4
64.3
CV%
6.4
5.1
3.3
9.2
7.4
6.5
Recovery:
The recovery of porcine IL-12/IL-23 p40 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=5)
99
88-109
Heparin Plasma (n=5)
94
85-105
Cell Culture Supernates (n=4)
104
97-113
EDTA Plasma (n=4)
92
84-99
Linearity:
To assess the linearity of the assay, samples spiked with or containing porcine IL-12/IL-23 p40 in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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