Long Name: Triggering Receptor Expressed on Myeloid Cells 1
Aliases: CD354
Entrez Gene IDs: 54210 (Human); 58217 (Mouse)
Background: TREM Family
The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.
原厂资料：
Product Summary:
The Quantikine Mouse/Rat TREM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse or rat TREM-1 in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse TREM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural TREM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring TREM-1.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Supernates (n=5)
98
87-109
Rat Cell Culture Supernates (n=5)
97
90-109
Mouse Tissue Homogenates (n=2)
100
91-104
Rat Tissue Homogenates (n=2)
99
95-103
Mouse Serum (n=5)
98
81-106
Rat Serum (n=4)
107
84-120
Mouse EDTA Plasma (n=6)
99
90-108
Rat EDTA Plasma (n=5)
100
85-112
Rat Heparin Plasma (n=5)
99
85-113
Mouse Heparin Plasma (n=5)
101
89-110
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TREM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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