Background:ST2
The Interleukin 1 receptor family comprises at least nine members. All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor) signaling domain that is also conserved in the Toll-like receptor family. A subtype of the IL-1 R family, SIGIRR (single Ig domain-containing IL-1 R-related molecule) differs from the other nine members by having only one Ig domain.
ST2L, also known as IL-1 R4, is a transmembrane glycoprotein in the interleukin-1 receptor family. ST2L is expressed on mast cells and Th2 cells as well as on stressed cardiac myocytes and airway epithelia. ST2L associates with IL-1R AcP to form a receptor complex for IL-33. Alternative splicing generates ST2, a secreted isoform that functions as an inhibitor of IL-33 signaling. Within the ECD, mouse ST2L shares 68% aa sequence identity with human ST2L.
原厂资料：
Product Summary:
The Quantikine Mouse ST2/IL-1 R4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ST2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse ST2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ST2.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=4)
103
89-113
Heparin Plasma (n=4)
94
86-103
Cell Culture Supernates (n=4)
98
81-111
EDTA Plasma (n=4)
104
92-118
Linearity:
To assess the linearity of the assay, samples containing high concentrations of mouse ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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