Mouse RBP4 Quantikine ELISA Kit (1 KT)

产品编号：RD-MRBP40 品牌：R&D 原厂货号：MRBP40
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: RBP4/Retinol-Binding Protein 4

Entrez Gene IDs: 5950 (Human); 19662 (Mouse); 25703 (Rat)

Background: Retinol-binding Protein 4/RBP4
Retinol (also known as vitamin A) is unstable and insoluble in aqueous solution. However, it becomes stable and soluble in plasma due to its tight interaction with RBP4, also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta-barrel structure with a well-defined cavity. It is secreted from the liver in a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, RBP4-retinol forms a complex with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.

原厂资料：

Product Summary:

The Quantikine Mouse RBP4 immunoassay is a 4.5 hour solid-phase ELISA designed to measure RBP4 in cell culture supernates, cell lysates, mouse serum, plasma, and urine. It contains NS0-expressed recombinant mouse RBP4 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse RBP4. Results obtained using natural mouse RBP4 showed dose response curves that were parallel to the standard curve obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RBP4.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	0.896	2.23	9.44	0.902	2.22	8.52
Standard Deviation	0.036	0.12	0.51	0.089	0.15	0.64
CV%	4	5.4	5.4	9.9	6.8	7.5

Recovery:

The recovery of mouse RBP4 spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	98	90-112
Urine (n=4)	91	80-107

Linearity:

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse RBP4 in each matrix were diluted with Calibrator Diluent and assayed.