Mouse Pentraxin 3/TSG-14 Quantikine ELISA Kit (1 KT)

产品编号：RD-MPTX30 品牌：R&D 原厂货号：MPTX30
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: Pentraxin 3/TSG-14

Long Name:Tumor Necrosis Factor Stimulated Gene 14

Aliases: PTX3; TSG-14

Entrez Gene IDs: 5806 (Human); 19288 (Mouse)

Background: Pentraxin 3/TSG-14
Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

原厂资料：

Product Summary:

The Quantikine Mouse Pentraxin 3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PTX3 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse PTX3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PTX3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PTX3.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	33	34	34
Mean	0.54	2.09	6.86	0.63	2.28	7.18
Standard Deviation	0.01	0.05	0.2	0.07	0.16	0.41
CV%	1.9	2.4	2.9	11.1	7	5.7

Recovery:

The recovery of mouse PTX3 spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=4)	102	90-117
Heparin Plasma (n=4)	95	81-100
EDTA Plasma (n=4)	98	90-110
Cell Culture Media (n=4)	107	98-119

Linearity:

To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse PTX3 in each matrix were diluted with Calibrator Diluent and assayed.