Background:Selectins
The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.
原厂资料：
Product Summary:
The Quantikine Mouse soluble P-Selectin (sP-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sP-Selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sP-Selectin and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse sP-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sP-Selectin.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
32
36
30
Mean
1.27
2.92
12.9
1.27
2.9
12.6
Standard Deviation
0.1
0.23
1.13
0.08
0.24
1.13
CV%
7.9
7.9
8.8
6.3
8.3
9
Recovery:
The recovery of mouse sP-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=10)
98
81-115
Cell Culture Supernates (n=4)
100
89-110
EDTA Plasma (n=6)
94
80-112
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sP-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
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