Background:Proprotein Convertases
This family of serine proteases consists of furin and proprotein convertases encoded by PCSK genes. They are important in a variety of processes. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. Mutations in the PC1/PCSK1 gene are associated with obesity and impaired prohormone processing.
原厂资料：
Product Summary:
The Quantikine Mouse PCSK9 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PCSK9 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant mouse PCSK9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PCSK9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PCSK9.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse PCSK9 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=6)
102
94-115
Cell Lysates (n=5)
103
91-117
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse PCSK9 in each matrix were diluted with Calibrator Diluent and then assayed.
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