Background:OSF-2/Periostin
OSF-2 (Osteoblast-Specific Factor 2), also known as Periostin, is a secreted, homodimeric protein that belongs to the periostin family of the FAS1 superfamily of molecules. It is a TGF-beta inducible molecule that serves as both an adhesion molecule and tumor suppressor. It is synthesized by smooth muscle cells, fibroblasts and osteoblasts, as well as in the periosteum and periodontal ligament. In bone, OSF-2 is thought to be involved in osteoblast recruitment, attachment and spreading.
原厂资料：
Product Summary:
The Quantikine Mouse Periostin/OSF-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Periostin in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse Periostin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Periostin. Results obtained using natural mouse Periostin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Periostin.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Periostin spiked to three levels throughout the range of the assay was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Samples (n=6)
95
81-117
Linearity:
To assess the linearity of the assay, samples containing high concentrations of mouse Periostin in each matrix were diluted with Calibrator Diluent and assayed.
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