Background:Endoglin
Endoglin, also known as CD105, is a type I integral transmembrane glycoprotein and is an accessory receptor for TGF-beta superfamily ligands. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages.
原厂资料:
Product Summary:
The Quantikine Mouse Endoglin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Endoglin in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Endoglin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural Endoglin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Endoglin.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Endoglin spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=4)
107
100-117
Heparin Plasma (n=4)
109
108-111
Cell Lysates (n=1)
115
-
EDTA Plasma (n=4)
107
99-114
Urine (n=4)
107
95-118
Cell Culture Samples (n=7)
102
93-114
Linearity:
To assess the linearity of the assay, four samples containing high concentrations of Endoglin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.