The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
原厂资料：
Product Summary:
The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
0.239
0.731
1.71
0.211
0.6
1.61
Standard Deviation
0.024
0.037
0.097
0.02
0.049
0.125
CV%
8.4
5.1
5.7
9.6
8.1
7.8
Recovery:
The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=4)
94
86-108
Linearity:
To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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