Background:CCL11/Eotaxin
Eotaxin is a potent eosinophil chemoattractant that is a member of the CC chemokine subfamily of inflammatory and immunoregulatory cytokines. At the protein sequence level, mature mouse Eotaxin is approximately 60% identical to mature human and guinea pig Eotaxin. Eotaxin is chemotactic for eosinophils, but not mononuclear cells or neutrophils.
原厂资料：
Product Summary:
The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves that were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Eotaxin spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=5)
98
82-111
Cell Culture Supernates (n=6)
96
82-103
Citrate Plasma (n=5)
97
81-114
EDTA Plasma (n=5)
100
83-118
Linearity:
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Eotaxin in each matrix were diluted with Calibrator Diluent and then assayed.
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