Mouse Lipocalin-2/NGAL Quantikine ELISA Kit (1 KT)

产品编号：RD-MLCN20 品牌：R&D 原厂货号：MLCN20
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: Lipocalin-2/NGAL

Long Name:Neutrophil Gelatinase-associated Lipocalin

Aliases: LCN2; NGAL; Siderocalin

Entrez Gene IDs: 3934 (Human); 16819 (Mouse); 170496 (Rat)

Background: Lipocalins
The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

原厂资料：

Product Summary:

The Quantikine Mouse Lipocalin-2/NGAL Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Lipocalin-2 in cell culture supernates, mouse serum, platelet-poor plasma, and urine. It contains NS0-expressed recombinant mouse Lipocalin-2 and antibodies raised against the recombinant factor. Natural mouse Lipocalin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural mouse Lipocalin-2.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	196	476	1,534	211	477	1,564
Standard Deviation	13.2	19.6	65.9	15.2	28.6	98.5
CV%	6.7	4.1	4.3	7.2	6	6.3

Recovery:

The recovery of mouse Lipocalin-2 spiked to three levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	93	82-101

Linearity:

To assess the linearity of the assay, samples containing high concentrations of mouse Lipocalin-2 were diluted with Calibrator Diluent and then assayed.