Background:Dkk Family
Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.
原厂资料：
Product Summary:
The Quantikine Mouse Dkk-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Dkk-1 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse Dkk-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Dkk-1. Results obtained using natural mouse Dkk-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Dkk-1.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Dkk-1 spiked to three levels throughout the range of the assay was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=4)
113
100-120
Heparin Plasma (n=4)
113
104-119
Cell Culture Supernates (n=4)
99
88-107
EDTA Plasma (n=4)
109
93-117
Linearity:
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Dkk-1 in each matrix were diluted with Calibrator Diluent and assayed.
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