Cotton rat growth-regulated protein (GRO) is a member of the ELR+ CXC subfamily of chemokines. The cotton rat GRO cDNA encodes a 100 amino acid (aa) precursor protein with a 27 aa putative signal peptide and a 73 aa mature protein.
The predicted amino acid sequence of the mouse KC gene product indicates KC is a member of the CXC subfamily of chemokines. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. In addition, the protein sequence of KC is approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs. KC mRNA is expressed in macrophages, endothelial cells, and fibroblasts.
The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha, CINC-2 beta and CINC-3 constitute a group of rat CXC chemokines that show significant sequence similarity to human GROs and mouse MIP-2 and KC.
原厂资料：
Product Summary:
The Quantikine Mouse KC Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse KC in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse KC and antibodies raised against the recombinant factor. This Immunoassay has been shown to quantitate the recombinant mouse KC accurately. Results obtained using natural mouse KC showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse KC.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
34.9
169
530
35
183
521
Standard Deviation
1.9
5.2
26.3
2.1
18
15.6
CV%
5.4
3.1
5
6
9.8
3
Recovery:
The recovery of mouse KC spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=7)
98
89-103
Serum (n=9)
95
83-104
Linearity:
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse KC in each matrix were diluted with Calibrator Diluent and then assayed.
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