MCP-1, also known as monocyte chemotactic and activating factor (MCAF), was initially purified based on its ability to chemoattract monocytes. Subsequent to its cloning and sequencing, it became evident that this protein is also identical to the product of the human JE gene. The JE gene, originally identified in mouse fibroblasts, is a platelet-derived growth factor (PDGF)-inducible gene.
原厂资料：
Product Summary:
The Quantikine Mouse/Rat CCL2/MCP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MCP-1 in mouse or rat cell culture supernates and serum. It contains E. coli-expressed mouse MCP-1 and antibodies raised against the recombinant factor. This Immunoassay has been shown to accurately quantitate recombinant mouse MCP-1. Results obtained using natural mouse or rat MCP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural MCP-1.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
47.1
311
768
43.6
310
783
Standard Deviation
3.9
15.8
40.6
3.2
14.3
42.8
CV%
8.3
5.1
5.3
7.3
4.6
5.5
Recovery:
The recovery of MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Supernates (n=6)
104
91-119
Rat Cell Culture Supernates (n=4)
109
103-115
Mouse Serum (n=9)
99
89-113
Rat Serum (n=4)
109
103-116
Linearity:
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MCP-1 in each matrix were diluted with Calibrator Diluent and then assayed.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
