Interferon-γ (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells.
IFN-γ was originally characterized based on its antiviral activities. The protein also exerts anti-proliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, up-regulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments Helper T cell expansion and may be required for Helper T cell differentiation.
IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-γ receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-γ receptor is structurally related to the IL-10 receptor.
原厂资料：
Product Summary:
The Quantikine Mouse IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IFN-gamma in cell culture supernates and mouse serum. It contains E. coli-expressed mouse IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IFN-gamma accurately. Results obtained using natural mouse IFN-gamma showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-gamma.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
44.8
156
518
43.3
144
509
Standard Deviation
2.2
5
14.2
3.6
14.7
48.6
CV%
4.9
3.2
2.7
8.3
10.2
9.5
Serum
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
45.1
143
528
42.8
139
518
Standard Deviation
2.1
4.4
11.8
3.6
13.6
46.5
CV%
4.7
3.1
2.2
8.4
9.8
9
Recovery:
The recovery of mouse IFN-gamma spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=5)
97
91-106
Cell Culture Supernates (n=7)
105
98-115
Linearity:
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IFN-gamma in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
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