Mouse Fas Ligand/TNFSF6 Quantikine ELISA Kit (1 KT)

产品编号：RD-MFL00 品牌：R&D 原厂货号：MFL00
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: Fas Ligand/TNFSF6

Aliases: CD178; CD95L; FASLG; TNFSF6

Entrez Gene IDs: 356 (Human); 14103 (Mouse); 25385 (Rat)

Background: Fas Ligand/TNFSF6

Fas Ligand is a 40 kDa type II transmembrane protein belonging to the TNF superfamily. The specific receptor for Fas Ligand is Fas (CD95, Apo-1). Fas Ligand is predominantly expressed on activated T cells and natural killer cells, while Fas is expressed on various types of cells. The Fas/Fas Ligand system plays a crucial role in modulating immune responses by inducing cell specific apoptosis. Human Fas Ligand shares 81% sequence identity with mouse Fas Ligand and 78% sequence identity with rat Fas Ligand.

原厂资料：

Product Summary:

The Quantikine Mouse Fas Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fas Ligand in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse Fas Ligand and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse Fas Ligand{\f9\i showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse Fas Ligand.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	84	261	675	76	243	663
Standard Deviation	8.1	9	20	6.8	12	33
CV%	9.6	3.4	3	8.9	4.9	5

Recovery:

The recovery of mouse Fas Ligand spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=6)	98	86-111
Cell Culture Supernates (n=6)	107	84-120
EDTA Plasma (n=6)	99	87-110

Linearity:

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse Fas Ligand in each matrix were diluted with Calibrator Diluent and then assayed.