FGF-21 is a 209 aa polypeptide that presumably has a signal sequence and is secreted. Human FGF-21 demonstrates only 75% aa identity with mouse FGF-21. In mouse, it is expressed preferentially in liver.
原厂资料:
Product Summary:
The Quantikine Mouse/Rat FGF-21 immunoassay is a 4.5 hour solid-phase ELISA designed to measure FGF-21 in mouse or rat cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse FGF-21 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse FGF-21. Results obtained using natural mouse or rat FGF-21 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural FGF-21.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
84.4
226
754
94.5
231
745
Standard Deviation
5.24
9.6
19.7
6.61
10.8
31
CV%
6.2
4.2
2.6
7
4.7
4.2
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
118
317
1,032
120
302
977
Standard Deviation
7.35
13.5
25.2
8.91
18
65.9
CV%
6.2
4.3
2.4
7.4
6
6.7
Recovery:
The recovery of FGF-21 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Mouse Cell Culture Samples (n=6)
96
88-101
Rat Cell Culture Samples (n=2)
91
87-95
Mouse Serum (n=4)
95
83-103
Rat Serum (n=4)
100
93-108
Mouse EDTA Plasma (n=4)
97
86-104
Rat EDTA Plasma (n=4)
101
93-119
Rat Heparin Plasma (n=4)
97
94-104
Mouse Heparin Plasma (n=4)
93
83-101
Linearity:
To assess the linearity of the assay, samples spiked with high concentrations of FGF-21 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.