Background:CXCL4/PF4 (Platelet Factor 4)
Platelet factor 4 (PF4) is a member of the CXC chemokine family. Human and mouse PF4 share 64% identity. PF4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity.
原厂资料：
Product Summary:
The Quantikine Mouse PF4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse PF4 in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse PF4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse PF4 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse PF4.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
0.23
0.41
1.51
0.25
0.45
1.46
Standard Deviation
0.013
0.029
0.046
0.019
0.035
0.11
CV%
5.7
7.1
3
7.6
7.8
7.5
Recovery:
The recovery of mouse PF4 spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=4)
102
90-120
Linearity:
To assess the linearity of the assay, samples containing high concentrations of mouse PF4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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