Fractalkine, also named neurotactin, is a chemokine identified through bioinformatics. Fractalkine has a unique CX3C cysteine motif near the amino-terminus and is the one member of a fourth branch of the chemokine superfamily. The extracellular domain of human Fractalkine can be released by proteolysis, at the dibasic cleavage site proximal to the membrane to generate soluble fractalkine.
原厂资料：
Product Summary:
The Quantikine Mouse Fractalkine Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse Fractalkine in cell culture supernates, tissue homogenates, mouse serum, and plasma. It contains E. coli-expressed recombinant mouse Fractalkine chemokine domain and antibodies raised against the recombinant protein. Results obtained for naturally occurring soluble mouse Fractalkine containing the chemokine domain and the mucin stalk domain showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Fractalkine.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Fractalkine spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Serum (n=6)
98
91-107
Heparin Plasma (n=6)
95
81-110
Cell Culture Supernates (n=5)
101
94-106
EDTA Plasma (n=6)
95
83-109
Tissue Homogenates (n=1)
100
100
Linearity:
To assess the linearity of the assay, samples containing or spiked with high concentrations of mouse Fractalkine in each matrix were diluted with Calibrator Diluent and assayed.
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