Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit (1 KT)

产品编号：RD-MCX130 品牌：R&D 原厂货号：MCX130
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: CXCL13/BLC/BCA-1

Aliases: BCA-1; BLC

Entrez Gene IDs: 10563 (Human); 55985 (Mouse)

Background: CXCL13/BLC/BCA-1
B cell-attracting chemokine 1(BCA-1), also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mature human BCA-1 shares 64% amino acid sequence identity with the mouse protein.

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Product Summary:

The Quantikine Mouse BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BLC/BCA-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse BLC/BCA-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BLC/BCA-1.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	51	109	408	52	104	366
Standard Deviation	3.1	3.8	15.4	5.1	7.1	27.1
CV%	6.1	3.5	3.8	9.8	6.8	7.4

Recovery:

The recovery of mouse BLC/BCA-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=7)	104	85-119
Heparin Plasma (n=4)	96	80-110
Cell Culture Supernates (n=4)	93	83-109
EDTA Plasma (n=7)	98	80-115

Linearity:

To assess the linearity of the assay, samples spiked with high concentrations of mouse BLC/BCA-1 in each matrix were diluted with Calibrator Diluent and then assayed.