Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit (1 KT)

产品编号：RD-MCX120 品牌：R&D 原厂货号：MCX120
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: CXCL12/SDF-1 alpha

Entrez Gene IDs: 6387 (Human); 20315 (Mouse); 493806 (Feline)

Background: CXCL12/SDF-1 (PBSF)
Stromal cell-derived factor (SDF)-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. They are encoded by a single gene, PBSF, and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. SDF-1/PBSF is highly conserved between species. Human mature SDF-1 shares approximately 99% amino acid sequence identity with the mouse protein.

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Product Summary:

The Quantikine Mouse SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SDF-1 alpha in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant mouse SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SDF-1 alpha.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean	1.09	2.6	4.1	1.25	3.15	4.91
Standard Deviation	0.04	0.12	0.21	0.09	0.24	0.37
CV%	3.7	4.6	5.1	7.2	7.6	7.5

Recovery:

The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	101	98-104

Linearity:

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.