Rat/Mouse Cytochrome c Quantikine ELISA Kit (1 KT)

产品编号：RD-MCTC0 品牌：R&D 原厂货号：MCTC0
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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描述：

Molecule Information: Cytochrome c

Aliases: CYCS

Entrez Gene IDs: 54205 (Human)

Background: Cytochrome c
Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.

原厂资料：

Product Summary:

The Quantikine Rat/Mouse Cytochrome c Immunoassay is a 2.5 hour solid phase ELISA designed to measure rat and mouse cytochrome c. It contains natural rat cytochrome c and antibodies raised against cytochrome c, and because of the 100% sequence identity of rat and mouse cytochrome c, this kit can be used to accurately quantitate natural rat or mouse cytochrome c in cell lysates and subcellular fractions.

Precision:

Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Lysates, Subcellular Fractions

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean	1.61	8.82	13.4	1.72	8.91	13.5
Standard Deviation	0.02	0.09	0.19	0.06	0.31	0.59
CV%	1.24	1.02	1.42	3.49	3.48	4.37

Recovery:

The recovery of rat/mouse Cytochrome c spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
PBS/0.01% Saponin (n=2)	94	90-98
PBS/0.05% Tween 20 (n=2)	101	100-102
PBS/0.5% Triton X-100 (n=2)	100	96-106
PBS/1% Triton X-100 (n=2)	98	95-101

Linearity:

To assess the linearity of the assay, five or more samples spiked with various concentrations of rat/mouse Cytochrome c in each matrix were diluted with Calibrator Diluent and then assayed.