Background:Clusterin
Clusterin (named for its ability to aggregate, or cluster, cells) is an 80-kDa, secreted, heterodimeric glycoprotein that has no identifiable structural motif(s). It is generated from a common precursor that is cleaved to form two disulfide-linked subunits. Clusterin is believed to function as a unique type of heat shock protein, presumably protecting extracellular molecules. Alternative splicing will also generate a truncated form of Clusterin that is pro-apoptotic. Thus, its function(s) may be context-dependent.
原厂资料：
Product Summary:
The Quantikine Mouse Clusterin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Clusterin levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Clusterin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Clusterin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Clusterin.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Clusterin spiked into various matrices was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Supernates (n=4)
101
90-111
Cell Lysates (n=4)
106
95-117
Linearity:
To assess the linearity of the assay, samples containing high concentrations of mouse Clusterin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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