Long Name:Retinoic Acid Receptor Responder Protein 2
Aliases:RARRES2; TIG-2
Entrez Gene IDs: 5919 (Human); 71660 (Mouse)
Background:Chemerin
Chemerin, also known as Tazarotene-induced Gene 2, (Tig-2), is a member of the Cystatin superfamily. Chemerin is synthesized as a 163 aa precursor that contains a hydrophobic 20 aa N-terminal sequence, an intervening 137 aa Cystatin fold-containing domain, and a six aa C-terminal prosegment. It is activated by proteases of the coagulation, fibrinolytic, and inflammatory cascades. Chemerin is a chemoattractant for cells bearing the Chem R23 receptor, including plasmacytoid dendritic cells and tissue macrophages.
原厂资料：
Product Summary:
The Quantikine Mouse Chemerin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Chemerin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature Chemerin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Chemerin. Results obtained using natural mouse Chemerin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Chemerin.
Precision:
Intra-Assay Precision (Precision within an assay)Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse Chemerin spiked to three levels throughout the range of the assay was evaluated.
Sample Type
Average % Recovery
Range %
Cell Culture Samples (n=4)
102
89-117
Linearity:
To assess the linearity of the assay, samples containing high concentrations of mouse Chemerin in each matrix were diluted with Calibrator Diluent and assayed.
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